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Acid-base indicators by I M Kolthoff

By I M Kolthoff

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The The 47 equilibrium between amino acids and hybrid ions. solution of residual amino an amino acid contains hybrid ions and the This equilibrium acid. may be described by: NH RCOOH * +NH RCOO-, 2 The value 3 n can be determined only approximately. For 4 and for dimethylglycine, glycine, and phenylalanine, n = 10 2 It is safe to assume that the unglycylglycine, n equals 10 dissociated portion of these amino acids is present as the hybrid of ; . ion. This is no longer true when the aromatic amino acids, such as benzoic acid derivatives, are concerned.

It has been shown that the strong acid-weak base type pH may of a solution of a salt of the be calculated in the following manner: pH = %pKw - |pBOH At ordinary temperatures %pKw equals i log 7, c. (35) whereas at 100 it is pH at 100 is smaller by one unit, equal to 6. Evidently the the of the and reaction solution at this temperature is corre- spondingly more acid. Conversely, in a solution of a salt of a strong base and weak acid, the hydroxyl ion exponent at 100 is likewise lowered to the same extent.

Any pH may be obtained simply by mixing a weak acid or base with one of its salts in various proportions. It is evident from equation (48) that even small amounts of strong acids and bases have only a slight effect on the pH of such Solutions of desired Certainly the small quantities of alkali from glass and carbon dioxide from the atmosphere can exert no perceptible mixtures. influence. Such mixtures which are resistant to a change in reaction were called Buffer Mixtures by S. P. L. SoRENSEN. 1 L.

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