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Advances in Biochemical Engineering - Biotechnology. - by Bernhard Sonnleitner

By Bernhard Sonnleitner

With contributions through a variety of specialists

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Extra info for Advances in Biochemical Engineering - Biotechnology. - Bioanalysis and Biosensors for Bioprocess Monitoring

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These aspects are not restricted to samples from monoseptic bioprocesses, they are equally important for environmental analyses [127]. Membranes do not only serve as the analytical interface. Schneider et al. [377] have demonstrated that hydrophobic membranes, for example, made from PTFE, mounted either in situ or in bypass, can also be used for preparative removal of ammonium from an animal cell culture. 3 Flow Injection Analysis (FIA) Ruzicka and Hansen [359] characterized flow injection analysis (FIA) as: “… information gathering from a concentration gradient formed from an injected, well-defined zone of a fluid, dispersed into a continuous unsegmented stream 26 B.

26). Useful fields are gravity, temperature, cross flow, electrical charge, and others [128–131]. The range of the (molecular) size of the analytes usually exceeds that which can be determined by classical laboratory analytical methods such as size exclusion chromatography, etc. [351]. Reports on investigated substances are widespread and cover applications such as the separation and characterization of proteins [450] and enzymes [240, 241], of viruses [132], the separation of human and animal cells [50, 51], the isolation of plasmid DNA [367], and the molecular weight and particle size distribution of polymers [216, 217].

27. g. g. by trypsinization) is necessary [117, 165]. g. [224], or in a flow cytometer [153]. Inactivation is not a decisive factor unless the population to be quantified is highly synchronized and close to the cell cycle phase of cell separation. In a few cases, biomass is estimated as colony forming units (cfu’s); however, this method is employed nearly exclusively in hygienic analyses of water, waste water, soil and sludge samples. An appropriately diluted, concentrated or suspended homogeneous aliquot of the sample is plated on solid medium and incubated for a predetermined time.

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