Skip to content

Affinity Chromatography by Amersham Biosciences

By Amersham Biosciences

Show description

Read Online or Download Affinity Chromatography PDF

Similar analytic books

Chromatographic Analysis of Pharmaceuticals

Up to date and revised all through. moment version explores the chromatographic tools used for the dimension of gear, impurities, and excipients in pharmaceutical preparations--such as capsules, ointments, and injectables. features a 148-page desk directory the chromatographic facts of over 1300 medications and similar substances--including pattern matrix analyzed, pattern dealing with tactics, column packings, cellular part, mode of detection, and extra.

Handbook of Vibrational Spectroscopy

THE DEFINITIVE source the 1st really accomplished paintings on vibrational spectroscopy, delivering a one-stop reference for infrared, near-infrared and Raman spectroscopy. AUTHORITATIVE, . .. With contributions from stated leaders within the box, the calibre of the editors and authors speaks for itself.

Cyclic voltammetry: simulation and analysis of reaction mechanisms

Cyclic Voltammetry is the one booklet completely dedicated to its topic and containing a knowledge research undertaking written by means of the writer. starting with the basics of cyclic voltammetry from either an experimental and theoretical viewpoint, the writer specializes in the functions in info interpretation with emphasis on chemical reactions and electrode relief potentials.

Guide to Fluorine NMR for Organic Chemists

Following its well-received predecessor, this ebook deals a necessary advisor to chemists for figuring out fluorine in spectroscopy. With over one thousand compounds and a hundred spectra, the second one version provides new facts – that includes fluorine results on nitrogen NMR, chemical shifts, and coupling constants. •    Explains easy methods to effectively include fluorine into objective molecules and make the most of fluorine substituents to structurally signify natural compounds•    Includes new facts on nitrogen NMR, targeting N-15, to painting the effect of fluorine upon nitrogen NMR chemical shifts and coupling constants•    Expands on every one bankruptcy from the 1st variation with extra info and up to date dialogue from fresh findings•    "The faultless ordering of fabric lined during this stand-alone quantity is such that details are available conveniently.

Extra resources for Affinity Chromatography

Example text

GSTrap FF columns can be used with a syringe, a peristaltic pump or a chromatography system. Enzyme-specific recognition sites are often included to allow the removal of the GST tag by enzymatic cleavage when required. Thrombin is commonly used for enzymatic cleavage, and must, subsequently, be removed from the recombinant product. HiTrap Benzamidine FF (high sub) 1 ml or 5 ml columns provide simple, ready-made solutions for this process (see page 54). Reuse of GSTrap FF depends on the nature of the sample.

Recombinant fusion proteins The purification of recombinant proteins can often be simplified by incorporating a tag of known size into the protein. As well as providing a marker for expression and facilitating detection of the recombinant protein, an important role for the tag is to enable a simple purification by affinity chromatography. The two most commonly used tags are glutathioneS-transferase (GST) and 6 x histidine residues (His)6. Protein A fusion proteins have also been produced to take advantage of the affinity between IgG and protein A for affinity purification.

3. Wash with 5–10 column volumes of the binding buffer to remove impurities and unbound material. Continue until no protein is detected in the eluent (determined by UV absorbance at 280 nm). 4. * 5. Immediately re-equilibrate with 5–10 column volumes of binding buffer. 0 per ml fraction), so that the final pH of the fractions will be approximately neutral. 35 Table 3 gives examples of some typical binding and elution conditions that have been used with Protein A Sepharose. Table 3. 0 3–4 (using thiocyanate) Binding strengths are tested with free protein A.

Download PDF sample

Rated 4.23 of 5 – based on 8 votes