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Analytical Biotechnology by C. van Dijk (Eds.)

By C. van Dijk (Eds.)

ANABIOTEC '92 involved in the additional integration of biotechnology and analytical chemistry. the result of this symposium in actual fact confirmed colossal development may be said within the program of either traditional and new analytical suggestions, the latter primarily in keeping with traditional analytical instruments reminiscent of biomolecules. the most topics coated in this assembly are fermentation tracking, chromatography, instrumental research, biosensors and bioanalysis

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16 T. Linden and B. Hahn-Hägerdahl, Enzyme Microb. , 11(1989)583. 17 G. Marko-Varga, L. Gorton, E. Domínguez and D. Barceló, Chromatographia, in press. A. J. O. A. Marko-Varga, Anal. , submitted for publication. C. van de Merbel, H. Th. Brinkman Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HVAmsterdam (Netherlands) A. C. de Rijke Pharmacia LKB Biotechnologie Nederland, Houttuinlaan 4, 3447 GM Woerden (Netherlands) (Received 1st October 1992; revised manuscript received 15th January 1993) Abstract The efficient control of fermentation processes requires reliable monitoring systems.

The value of this parameter mainly depends on the dead volume of the filtration module and the filtrate flux through the membrane. Therefore, to obtain a fast response, both parameters should be optimized. The flux through a membrane is described by [17]: /= ΔΡ 1 = ΔΡ 1 (1) 8d where d is the membrane thickness (m), n the number of pores per unit area, A the membrane area (m 2 ) and r the pore radius (m). Eqns. 1 and 2 demonstrate that to obtain a maximum flux a proper construction of the filtration module is essential.

Acta 279 (1993) 39-50 3\Δ Fig. 2. Schematic drawing of the hollow-fibre ultrafiltration module. Only two hollow fibres are shown, the hatched areas indicate the potting material used to seal the fibre bundle in the housing. The sample is pumped through the dead volume of the module, the filtrate is collected within the fibres. During filtration one of the filtrate outlets is closed. 4 ml min - 1 ). d. stainless-steel reaction coil at 90°C. The signal was recorded by a Model 2210 TABLE l Time schedule of the on-line analytical procedure using the system depicted in Fig.

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