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Atherosclerosis: Experimental Methods and Protocols (Methods by Angela F. Drew

By Angela F. Drew

In Atherosclerosis: Experimental tools and Protocols, Angela Drew and a panel of specialists have assembled a entire selection of conventional and state of the art recommendations for investigating this ailment and its attainable remedies. every one quite simply reproducible technique comprises step by step directions and functional information about pattern assortment, the alternative of animal version procedure, experimental layout, and functional info research suggestions. complete and richly precise, Atherosclerosis: Experimental equipment and Protocols allows all biomedical investigators to pick these optimized innovations which may be so much fruitfully used to review the improvement, development, and remedy of atherosclerotic lesions this day.

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Extra info for Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine)

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E. and Vance, J. ),Elsevier, New York, pp. 403–426. 6. Havel, R. J. and Kane, J. P. (1995) Introduction: Structure and metabolism of plasma lipoproteins, in The Metabolic and Molecular Basis of Inherited Disease, vol. 2, (Scriver, C. , Beaudet, A. , Sly, W. , New York, pp. 1129–1138. 7. Mackness, M. I. and Durrington, P. N. (1992) Lipoprotein separation and analysis for clinical studies, in Lipoprotein Analysis: A Practical Approach (Converse, C. A. and Skinner, E. ), Oxford University Press, New York, pp.

7. 8. 9. All other chemicals or reagents not specified otherwise above are from Sigma Chemical Co. (St. Louis, MO), including cholesterol, triglyceride, and protein assay kits. Phospholipid assay kits are from Boehringer Mannheim (Germany). 04%). Adjust the solvent density (see Table 2) of the sample as determined by the equation above or by the addition of solid NaBr (see Table 3) to isolate the lipoproteins of interest. 006 g/ mL, VLDL and chylomicron particles are isolated. 21 g/mL, HDL particles are isolated.

6. Havel, R. J. and Kane, J. P. (1995) Introduction: Structure and metabolism of plasma lipoproteins, in The Metabolic and Molecular Basis of Inherited Disease, vol. 2, (Scriver, C. , Beaudet, A. , Sly, W. , New York, pp. 1129–1138. 7. Mackness, M. I. and Durrington, P. N. (1992) Lipoprotein separation and analysis for clinical studies, in Lipoprotein Analysis: A Practical Approach (Converse, C. A. and Skinner, E. ), Oxford University Press, New York, pp. 11–43. 8. Wasan, K. , Cassidy, S. , Strobel, F.

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