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The benzene is removed in vacuo, ethylene glycol is removed by extraction with H20/ethyl acetate to yield a white solid. 00 (m, 38 (a) A. A. Amos and P. Ziegler, Chem. Ber. 37, 345 (1959); and (b) R. E. Ireland and D. M. Walba, Org. Syn. Coll. 6, 567 (1988). 02; IR (CHCI3) 3280, 1610, 1512, 1455, 1273, 1168, 1002 cm -1. 1416. 1400. 52. 49. p-Hydroxyphenacyl Diammonium Phosphate (16). 27 The hydrogenolysis is performed according to the procedure of Heathcock. 1 mmol) dissolved in 30 ml of methanol is added 186 mg of 10% Pd/C.
Exposures of longer duration than 10 msec generate sufficient glutamate to cause a regenerative C a 2+ spike, as indicated by the large jump in the response. Figure 4 shows similar photorelease studies but in voltage-clamp recordings. Currents as large as 1500 pA are observed. These studies were all conducted in the presence of tetrodotoxin (TTX) to block regenerative sodium action potentials. Summary This chapter introduces two new phototriggers for caging nucleotides, amino acids, peptides, and related functional group derivatives, potentially including higher-order homologs.
04 M at pH 6. 6-nm C18 reversed phase column. 5 ml/min. Photolysis is performed on a merry-go-round apparatus equipped with 16 x 300-nm or 4 x 350-nm lamps. The light output for the determination of quantum yields is measured using the potassium ferrioxalate method, an Into a 20 x 180-mm Pyrex tube is placed 5 or 10 ml of an aqueous or alcoholic solution containing the phosphate ester and an appropriate internal standard. , greater than 3 absorbance 44 C. G. Hatchard and C. A. Parker, Proc. R. Soc.